TABLE 3.

Oligonucleotides used for TaqMan sqRT-PCR LGI1 gene expression experiments

08-5578 LGI1 target gene	Sequence (5′–3′)			Length of PCR product (bp)
	Forward primer	TaqMan probe	Reverse primer
bglAa	AGCTGCTGCTGCAAACCAAT	CGAAGGCGCTTACAACGTCGATGG	AGTAACATCTTGAACGGAAAGTCCTT	73
rRNA genea	CTTCCGCAATGGACGAAAGT	TGACGGAGCAACGCCGCGT	TTACGATCCGAAAACCTTCTTCA	66
lmo1901	TGATCCGCCGTATTACGAAAC	AGGGTCACTACGCTGTGGTGTTCCGA	AAGCCGTGCATGATCTTCCT	70
lmo1891	ACGGCAGGCAGTGGTTATG	TGCCAACACAGGTGAGCAGGAAGA	AAGGCAGTTCCTTTTCCTCAGA	67
lmo1889	GGCGGGAAAACCGAGAAG	AACACTTTGACAGCCCAGAACACGCC	TGCGGCGTACCACATTGA	70
lmo1885	CGGCGATGTGGTGGAATAC	TGTCGGGCTTGGAGGTAACAGTT	GAGCCAGCGGAGCATTTC	65
lmo1883	TTCACCTCACTATGCTCCACTACTG	CTATGCGGTTGGTGACGGGAATGTAGA	CATTCCACCACCGCCATT	72
lmo1882	CCGCTTTTGCAGCAGGAA	AGGCGATGTTGCCGGAGCGATT	GGGATGCAGTCGTCCATGTC	66
lmo1876	CACTTACCGCTGCGTTTGG	CGCTCCGAGAATACCCGACTGCTACA	TGTCGAAGAATTGCCTGCTCTT	69
lmo1873	ACAGCATTCGTATGAAAGCCATT	TTTACGCTTTGTTTTTCGGCACGGAA	TCGTCAGCGTTCATTTTGAGA	73
lmo1866	CACGTCATTAGTCTTCGCAGAGA	ATGCTGCCCGCCTTGGATATGACA	ATTAAATCCTGCCATTGCTTTCC	74
lmo1865	AGTTCCTATCCCTGCGGAAGA	CATCCACAGCTTATGCTTCGGGATGAA	TTGGGATACTTGCCCGCATA	70
lmo1864	ACCGGGAGATGCTGACAATT	CTGATGAATACGGTGGCAAGCTGCC	CTGCGAAAATCATCACACGATT	69
lmo1863	TCTCCCGCAGTACCGTGAA	CGTGCCATTGCTGACCTTGAA	CCTTGGAGAGATGGCCAGTCT	63
lmo1862	AAATGTCTGATGGACTTACAAAGCTATT	CCAAGTGCAGGGATGTTCATAGCATTTATCCTA	GCACTAACCGGTATTTTTTTTAATGC	109
lmo1861	GGAAATAGAAGCTTTCGCCAGTAT	CGCAGGAACAACGTAGGGCTATATCCG	GGAACTCCCGAGAAAAGAAAATG	79
lmo1859	AAGAGCGCGAAGCTGAAAGATA	AAGGAAGTGCATTCATCAATTTGAGCTTTCC	CCTCATCTTGGAATCGTTCCA	78
lmo1857	CACTGCCGCCAAGAAAAAAA	CCAGCTTCTTGACAGTAATGGTTTTGACCGA	GCAACTCTGTCCGAGCAATTT	77

^aHousekeeping gene not carried on LGI1. The relevant primers were described by Tasara and Stephan (38).