TABLE 4.

Oligonucleotides used for qRT-PCR experiments^a

Primer	Oligonucleotide sequence (5′-3′)	Product size (bp)	Primer efficiency (%)
16S rRNA-Fb	TTA GCT AGT TGG TAG GGT	318	91.3
16S rRNA-Rb	AAT CCG GAC AAC GCT TGC
emrE-JKq-F	GTT GCT ATA GCG GTG ATT GGA GT	102	104.3
emrE-JKq-R	GTT CAG GCC TAC AAC CCC TG
lde-JKq-F	TCC CAA TGG CTT TCG CAC AA	136	99.4
lde-JKq-R	ATT CGA CCT GCA ACC TCA CG
lmo1861-JKq-F	GCT TAC AGA AGA AGG AGC GCA	101	99.6
lmo1861-JKq-R	CCC TAC GTT GTT CCT GCG G
mdrL-JK2q-F	TCG AGC TGG TTG GGG TTT TG	96	97.1
mdrL-JK2q-R	ATC CCA ATT GCA TGG CCT GG
sigBq-Fc	TGT TGG TGG TAC GGA TG	221	100
sigBq-Rc	CAT TCT GCA ACG CCT C

^aAll primers were synthesized by Integrated DNA Technologies, Inc. (Coralville, IA).

^bPrimers validated by Tasara and Stephan (38).

^cPrimers designed by Arguedas-Villa et al. (19).