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Sequential multihost isolation method B. (1) Phage stock is added to host 1 at exponential phase. (2) Phages infecting host 1 are adsorbed by host 1. (3) Free phages and adsorbed phages are separated by centrifugation. Phages infecting host 1 are enriched afterwards. (4 to 6) Enriched phages are added to host 2 at exponential phase, and the previous procedures are repeated with host 2. (7) Phages enriched by the last host are isolated by using the double-layer plate assay.
