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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Cell Biochem. 2015 Sep 17;117(3):769–779. doi: 10.1002/jcb.25362

Fig. 3.

Fig. 3

H2O2 treatment does not alter occludin protein content in whole cell lysate, Triton X-100-soluble lysate, or Triton X-100-insoluble lysate. Western blot analysis of the effect of 2 h treatment of MDCK cell monolayers without or with 55 μM H2O2 on occludin protein content in the total cell lysate, the Triton X-100-soluble lysate fraction, and the Triton X-100-insoluble lysate fraction. Western blot analysis of ERK ½ protein content in the total cell lysate and Triton X-100-soluble lysate fraction are shown for comparison.