. Author manuscript; available in PMC: 2017 Mar 1.

Published in final edited form as: J Cell Biochem. 2015 Sep 17;117(3):769–779. doi: 10.1002/jcb.25362

TABLE I.

Treatment With H₂O₂ Slows the Movement of Full-Length GFP-Occludin Protein, but not C-Terminal-Truncated GFP-Occludin Protein Into the Tight Junction Region of Post-Confluent MDCK Cells

	[H₂O₂] (μM)	Recovery halftime (t_1/2)(s)	Immobile fraction (%)	Number of replicates (n)
Full-length GFP-occludin	0	51.45 ± 10.53	45.7 ± 8.1	10
	55	77.57 ± 10.57^**	36.6 ± 13.7	16
Truncated GFP-occludin	0	56.27 ± 18.74	27.2 ± 15.0	42
	55	61.16 ± 18.24	25.3 ± 9.1	37

FRAP analysis was performed on post-confluent MDCK cell populations expressing either full-length GFP-occludin protein or C-terminal-truncated GFP-occludin protein. Prior to FRAP analysis, cell populations were treated without or with 55 μM H₂O₂ for 2 h. Derived parameters, t_1/2 and % Immobile Fraction, were calculated for each experiment. Results are presented as mean ± SD of derived parameters for the indicated number of independent experiments. Results are representative of studies using at least three separate cell populations.

P < 0.05 compared to 0 μM H₂O₂

^**

P < 0.01 compared to 0 μM H₂O₂.