. Author manuscript; available in PMC: 2017 Mar 1.

Published in final edited form as: J Cell Biochem. 2015 Sep 17;117(3):769–779. doi: 10.1002/jcb.25362

TABLE II.

Treatment With H₂O₂ Does not Slow the Movement of Full-Length GFP-Occludin Protein Within the Apical Membrane of Post-Confluent MDCK Cells

[H₂O₂] (μM)	Recovery halftime (t_1/2) (s)	Immobile fraction (%)	Number of replicates (n)
0	51.68 ± 18.23	28 ± 11	12
55	45.21 ± 21.11	34 ± 17	13

FRAP analysis was performed on post-confluent MDCK cell populations expressing full-length GFP-occludin protein. Prior to FRAP analysis, cell populations were treated without or with 55 μM H₂O₂ for 2 h. Derived parameters, t_1/2 and % Immobile Fraction, were calculated for each experiment. Results are presented as mean ± SD of derived parameters for the indicated number of independent experiments. Results are representative of studies using at least two separate cell populations.

P < 0.05 compared to 0 μM H₂O₂

^**

P < 0.01 compared to 0 μM H₂O₂