Fig. 2.

The infusion of 10 µl autologous whole blood into the striatum of mice produces extensive brain damage and severe neurologic deficits within 72 h. Age- and weight-matched C57BL/6 mice were randomly allocated into two groups: intracerebral hemorrhage group (ICH) and needle insertion only group (sham). (A) Representative brain sections obtained 72 h after the sham procedure (left) and intrastriatal infusion of 10 µl autologous whole blood (right). Brain sections were stained with Luxol fast blue/Cresyl Violet. Intrastriatal infusion of 10 µl blood produced extensive brain damage; whereas the sham procedure produced very limited needle track damage. Scale bar = 100 µm. (B) Quantification shows significantly larger brain injury volume in the ICH group than in the sham group at 72 h (n = 7/group, **p < 0.001). (C) An investigator blinded to the experimental cohort assessed the neurologic deficits of mice with a 24-point neurologic scoring system at 4, 24, 48, and 72 h. Neurologic deficits were significantly more severe in the ICH group than in the sham group (n = 8/group, **p < 0.001). Values are means ± SD.