Fig. 1.

Ionic current development and Mn²⁺-induced fluorescent quench in HEK293-overexpressing mouse TRPM7. (A) Average time course of current development assessed in TRPM7-HEK293 using the whole-cell configuration of the patch-clamp technique (n = 10). Error bars indicate SEM. Note different scale for positive and negative currents. (B) Current-voltage (I/V) curve extracted from an example cell at 300 s into the whole-cell experiment. (C) Fura-2 quenching in TRPM7-HEK293 cells (positive control, blue circles) and noninduced TRPM7-HEK293 cells (negative controls, black circles) that were plated at 60,000 cells/well. The fura-2 loading buffer contained 2 µM fura-2-AM, 2 mM probenecid, and 0.1% pluronic F-127 in KRH, and incubation was at 37 °C for 60 min. Each data point is the mean ± SD of 48 replicate wells for TRPM7-HEK293 cells and 24 replicate wells for noninduced TRPM7-HEK293 cells, and Z′ factor values (red triangles) are plotted.