Fig. 1.

MCH phenotypes associated with CHES-1-like, jumu and htl. (A-J) Mef2 antibody staining of CCs, illustrating the presence or absence of the MCH phenotype (square brackets) in representative Stage 16 embryos that are (A) wild type, (B) homozygous for the CHES-1-like null mutation, (C) homozygous for the jumu null deficiency, (D,E) doubly homozygous for both the CHES-1-like mutation and the jumu deficiency, (F) doubly homozygous for both the CHES-1-like mutation and the jumu deficiency, but ubiquitously expressing polo, (G) expressing a dominant-negative version of the Htl FGFR pan-mesodermally, (H) heterozygous for a htl null mutation, (I) homozygous for the CHES-1-like null mutation and heterozygous for the htl null mutation, or (J) homozygous for the jumu null deficiency and heterozygous for the htl mutation. Scale bar: 50 μm. (K) Quantification and significance of the MCH phenotypes. From left to right, the P-values indicate the significances of the difference in phenotype between embryos lacking both Fkh genes and embryos lacking either the CHES-1-like or jumu gene; the difference in phenotype between embryos missing both Fkh genes and embryos missing both Fkh genes while ubiquitously expressing polo; the difference between the phenotype of embryos both heterozygous for the htl null mutation and homozygous for the CHES-1-like null mutation and the additive effects of the phenotypes in htl heterozygotes and the CHES-1-like homozygotes; and the difference between the phenotype of embryos both heterozygous for the htl null mutation and homozygous for the jumu null deficiency and the additive effects of the phenotypes in htl heterozygotes and the jumu homozygotes.