Fig. 8.

Simultaneous mesoderm-targeted ectopic expression of both Htl and Fz reduces the severity of the MCH phenotype in embryos lacking both CHES-1-like and jumu functions to wild-type levels. (A-C) Mef2 antibody staining of CCs, illustrating the presence or absence of the MCH phenotype (square brackets), in representative Stage 16 embryos lacking both functional CHES-1-like and jumu genes but possessing (A) twi-GAL4, (B) UAS-htl and UAS-fz1-1, or (C) twi-GAL4, UAS-htl and UAS-fz1-1 transgenes, and therefore simultaneously expressing Htl and Fz constitutively throughout the entire mesoderm. Scale bar: 50 μm. (D) Mef2 antibody staining of CCs in a representative Stage 16 wild-type embryo. (E) Quantification and significance of the MCH phenotypes.