Fig. 2.

SoxC is required for the differentiation of all neurons. (A-C) Immunostaining for serotonin (green) and the pan-neural marker SynB (red) is greatly reduced when SoxC is knocked down by morpholino injection (A, control; B and C, two morphants). (D-F) SoxC (red) and SynB (green) transcripts are expressed in different cells (D, overlap of two channels; E and F, individual channels). (G-I) SoxC-expressing and SynB-expressing cells both form clusters in embryos treated with DAPT, but labeling for the two markers is mutually exclusive. Nuclei are labeled with DAPI (blue). All embryos are at 68-70 h and shown with the anterior pole at the top. Scale bar: 20 µm.