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. 2016 Jan 15;143(2):298–305. doi: 10.1242/dev.125989

Fig. 6.

Fig. 6.

The Brn1/2/4 overexpression neural phenotype requires SoxC function. Embryos were injected with SoxC morpholino or Brn1/2/4 synthetic mRNA alone or in combination and immunostained for serotonin (green) or SynB (red). (A) A control embryo. (B) A SoxC morphant that lacks all neurons. (C) An embryo overexpressing Brn1/2/4 mRNA (higher dose) shows expanded labeling for SynB and lacks serotonergic neurons. (D) Injection of Brn1/2/4 mRNA does not rescue SynB-expressing neurons in the SoxC morphant. Nuclei are labeled with DAPI (blue). All embryos are at 72 h. Scale bar: 20 µm.