Fig. 7.

Knockdown of duf and rst during development does not lead to defects in filament organization in adult reproductive muscles. Filament arrangement of (A,C) wild-type and (B,D) duf-RNAi;rst-RNAi double knockdown in (A,B) smooth-like testes muscles and sarcomere pattern of (C,D) multinuclear sperm pump muscles. F-Actin (red) was visualized with Phalloidin; Hoechst dye (white in A and B; blue in C and D) was used to label DNA in nuclei. In C and D, anti-Mef2 antibody (green) was used to detect muscle nuclei. All photographs are optical sections. Scale bars: 20 µm.