Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 13;6:18948. doi: 10.1038/srep18948

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a–e) Two hit categories were established by ranking topographies, as shown in Fig. 3a: the 100 topographies (TopoUnits) with the highest (Top 100) and lowest (Bottom 100) number of Oct4+ cells at 24 h. For each category the total number of attached cells at 4 h (a) and the number of Oct4+ and EdU+ cells per topography at 24 h (b) were quantified and compared with a flat topography (polystyrene). % Oct4+ (c) and % EdU+ (d) cells were also plotted against total number of adherent cells. (e) The number of clusters and cells per cluster are shown for each category. *p < 0.05; **p < 0.01; ***p < 0.001; ns: not significant. 25% confidence intervals are shown. (f–h) Individual TopoUnits representing one of the bottom (f) or top (h) 100 topographies are shown, with flat polystyrene (g) for comparison. (i–k) Colony formation at 4 days on topographies that supported EdU+ and Oct4+ cells at 24 h. Scale bars: 55 μm.