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. 2016 Jan 13;6:19395. doi: 10.1038/srep19395

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Circular wounds of 1 mm in diameter were made using a punch template. The migration of individual human corneal epithelial cells in stratified cultures and in the presence of serum was analyzed by time-lapse imaging. (a) Cell-tracking experiments revealed that cells at the leading edge and stratification front migrated with an average trajectory speed of 0.27 and 0.17 μm/min respectively. (b) Following a wound, epithelial cells at the leading edge and stratification front healed by unified directional movement towards the wound area. Representative cell tracks in three wounds are shown. Tracks are color-coded and represent individual wounds.