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. 2016 Jan 19;110(2):362–371. doi: 10.1016/j.bpj.2015.12.013

Figure 4.

Figure 4

Phosphorylation extends and stiffens the test peptide at 0 M NaCl as measured by FRET. (A) Distance distributions of the semiflexible chain model, for threonine-containing peptides. Broken line, unphosphorylated; solid line, phosphorylated. (B) Distance distributions of the semiflexible chain model, for serine-containing peptides. Broken line, unphosphorylated; solid line, phosphorylated. (C) Model peptide mean end-to-end distances. Error bars represent 95% confidence intervals. Mean change: pThr = +2.24 Å; pSer = +1.83 Å. (D) Experimentally fit model peptide persistence lengths. Error bars represent 95% confidence intervals. Mean change: pThr = +2.86 Å; pSer = +2.22 Å. To see this figure in color, go online.