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. 2015 Nov 26;48(2):506–516. doi: 10.3892/ijo.2015.3268

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Copyright: © Nakayama et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Western blot analysis. (A) Western blot analysis showed that CCNE1 protein expression was higher in JHUC-1 and HEC108 cells than in other cell lines. (B) Western blot analysis showed that CCNE1 protein expression was significantly decreased in CCNE1 siRNA-transfected cells compared with control siRNA-transfected cells. (C) Effects of CCNE1 siRNA on cell proliferation. Cell numbers were determined at 72 h after transfection with CCNE1 siRNA or control siRNA. Endometrial carcinoma cells with CCNE1 overexpression were more sensitive to growth inhibition by CCNE1 siRNA than cells without CCNE1 overexpression. The mean and SD were obtained from three experiments.