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. 2015 Dec 10;48(2):624–634. doi: 10.3892/ijo.2015.3286

Figure 1.

Figure 1

Treatment of human melanoma cells with GSPs inhibits cell viability and induce apoptotic cell death. (A and B) A375 and Hs294t cells were treated with varying concentrations of GSPs for 24 and 48 h and cell viability was determined using the MTT assay, as described in Materials and methods. Cell viability is expressed in terms of percent of control (non-GSPs-treatment), as the mean ± SD, n=6–8. *P<0.01; P<0.001 vs. non-GSP-treated control group. (C) Melanoma cells, A375 and Hs294t, were treated with varying concentrations of GSPs (0, 20, 40 and 60 μg/ml) for 48 h, then harvested for the analysis of apoptosis using the Annexin V-Alexa Fluor 488 (Alexa 488) apoptosis vybrant assay kit. (D) The percentage of apoptotic cells in each treatment group summarized after 48 h of GSPs treatment. Data are presented as mean ± SD of three experiments. Significant difference vs. control group (non-GSP-treated). *P<0.01 and P<0.001. (E) Western blot analysis of pro-apoptotic and anti-apoptotic proteins in A375 and Hs294t cells.