Figure 2.

Effect of GSPs on β-catenin and its signaling molecules in human melanoma cells in vitro. (A) A375 and Hs294t cells were treated with varying concentrations of GSPs for 48 h. Cell lysates were subjected to the western blot analysis of different proteins of β-catenin signaling pathway. (B) Effect of GSPs on cell death of Mel928 cells (activated β-catenin) vs. Mel1011 cells (wt β-catenin) as analyzed by standard trypan blue staining. Cells were treated with GSPs for 24 and 48 h, then harvested. The number of dead melanoma cells were counted under a microscope. Resultant dead cells in each treatment group are expressed as a percentage of total number of cells/treatment group. Data shown are mean ± SD, n=3. ^*P<0.01; ^¶P<0.001 vs. non-GSP-treated control group.