Figure 6.

Effect of β-catenin manipulation on proliferation potential of melanoma cells. (A and B) The siRNA knockdown of β-catenin in Mel1241 cells (which have activated β-catenin) inhibits melanoma cell growth. β-catenin was knocked down in Mel1241 cells using siRNA transfection protocol. Forty-eight hours after the transfection of scrambled and β-catenin siRNA, Mel1241 cells (both control and β-catenin knock out) were harvested and plated in a 6-well plate (50,000/well). Cells were harvested at 24 and 48 h time-points and were counted under a microscope using hemocytometer. (A) Detection of β-catenin level in scrambled and siRNA knockdown of Mel1241 cells using western blotting. (C) Overexpression of β-catenin^S33Y in Mel1011 cells enhances the rate of proliferation of Mel1011 cells. Mel1011 cells lack activated β-catenin. Verification by western blot analysis. (D) Forced expression of β-catenin in Mel1011 cells significantly enhances its proliferation potential compared to scrambled-treated cells, ^*P<0.01, n=3.