Figure 7.
Effect of dietary GSPs on the growth of β-catenin activated and β-catenin inactivated melanoma tumor xenograft in athymic nude mice. Mel928 (activated β-catenin) and Mel1011 (β-catenin inactivated) cells were subcutaneously implanted in athymic nude mice and the effect of dietary GSPs was determined on weekly basis. (A) The volumes of tumor xenograft was recorded in each treatment group once per week. Average tumor volume (mm3) ± SD was plotted as a function of time. (B) Tumor xenograft tissues were harvested at the termination of the experiment at day 49, and wet weight of the tumor per mouse in each group is reported as mean ± SD (g), n=5. Significant difference vs. non-GSP-treated control group, ¶P<0.01. (C) Effect of dietary GSPs on the levels of PCNA and cleaved caspase-3 in the treatment groups of both Mel928 and Mel1011 tumor xenografts. Tumor lysates were used for western blot analysis.