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Silencing of LOXL2 by using si-LOXL2 in lung SCC cells. Silencing of LOXL2 mRNA and protein expression by si-LOXL2 transfection and the effects of the silencing of LOXL2 on SK-MES-1 and EBC-1 cell activities. (A) LOXL2 mRNA expression was evaluated by qRT-PCR 72 h after transfection with miR-29s. GUSB was used as an internal control. *P<0.0001. (B) LOXL2 protein expression was evaluated by western blotting 96 h after transfection. GAPDH was used as a loading control.
