Table I.
Sequences of RNA and DNA oligonucleotides.
| Name | Sense strand/sense primer (5′-3′) | Antisense strand/antisense primer (5′-3′) |
|---|---|---|
| Primers for RT-PCR | ||
| PIK3R1 | TTTGCCGAGCCCTATAACT | TGCATATACTGGGTAGGCTAGT |
| 18s rRNA | CCTGGATACCGCAGCTAGGA | GCGGCGCAATACGAATGCCCC |
| Primers for the 3′-UTR of PIK3R1 cloning | ||
| PIK3R1 XhoIF | ccgctcgagAGCGCTTACTCTTTGATCCTTCTCC | |
| PIK3R1 NotIR | CTATTAGGGTAGTGACCATATTATGGTTG | |
| mutPIK3R1-1 | GTTTTAAATGTACCTTCAGATATTCGATCCCCACCCCAGTTTTTGTT | AACAAAAACTGGGGTGGGGATCGAATATCTGAAGGTACATTTAAAAC |
| mutPIK3R1-2 | GTTTTGTTGGGCAGTGCCTGTATTCGATCAAAGCTGCTTTATTCAAT | ATTGAATAAAGCAGCTTTGATCGAATACAGGCACTGCCCAACAAAAC |
| siRNA duplexes | ||
| PIK3R1-siRNA1 | CAAAGGAUUAUGCAUAAUUdTdT | AAUUAUGCAUAAUCCUUUGdTdT |
| PIK3R1-siRNA2 | CCAAUAUUCACUGGUGGAAdTdT | UUCCACCAGUGAAUAUUGGdTdT |
| PIK3R1-siRNA3 | CUAUUGAAGCAUUUAAUCAdTdT | UCAUUAAAUGCUUCAAUAGdTdT |
| Control-siRNA | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
RT, reverse transcription primer; F, forward primer; R, reverse primer; 3′-UTR, 3′-untranslated region.