Fig. S1.

Mixing of liposome contents by the F/T method. (A) Experimental scheme of the content mixing assay by F/T. Two liposome solutions encapsulating different fluorescent content markers (L1:TA647 and L2:TA488) were mixed, centrifuged (mix), and subjected to F/T. Both liposomes were labeled with a fluorescent lipid marker (ATTO565). The fused liposomes possessed both TA488 and TA647 content markers. (B) The 2D plots of fluorescence measured by FCM. The horizontal axis shows the fluorescence of the TA488 content marker, and the vertical axis shows the fluorescence of the TA647 content marker. Liposomes containing both fluorescent content markers were constructed as a P.C. (C) Confocal microscopic images of liposomes before mixing (Upper, L1; Middle, L2) and after F/T (Lower, F/T). The fluorescence of TA488, TA647, and ATTO565 and the merged images are shown. (Scale bar, 25 μm.)