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. 2015 Dec 31;113(3):632–637. doi: 10.1073/pnas.1518079113

Fig. 3.

Fig. 3.

The TbBBSome is dispensable for viability but required for parasite virulence. (A) Western blot for TbBBS-HA proteins in TbBBS KO mutants or WT background as a positive control. Lysates from WT cells (i.e., without an HA tag) served as negative control. WT lanes are the same for BBS1-HA/BBS4-HA blots (*) and BBS5-HA/BBS7-HA blots (†). VSG was used as loading control. (B) Western blot for TbBBS-HA proteins in TbBBS KO mutants by blue native PAGE. WT lysates served as negative control. The same lysates were run in parallel and blotted for VSG on SDS/PAGE (A) to verify equal total protein. (C) Transmission electron micrographs of WT and TbBBS mutants at the flagellar pocket region. (D) Survival curve for mice infected with TbBBS mutants. Only mice in which parasitemia was detected are shown. Inset shows the Western blot for TbBBS4-HA by blue native PAGE. ns, Not significant. *P < 0.05 by Mantel–Cox test; ***P < 0.001 by Mantel–Cox test.