Fig. 2.

NCoR represses promoter activity of prometastatic genes. (A) Transient transfection assays in SK and SK-TRβ cells with luciferase reporters of the COX2, MMP9, and ID1 promoters or an empty plasmid (e.p). Schematics of the plasmids used showing the putative binding motifs for different transcription factors are illustrated. Cells were treated with and without 5 nM T3 for 36 h as indicated. Data are means ± SD and are expressed relative to the luciferase activity obtained in untreated SK cells. (B) ChIP assays in SK and SK-TRβ cells with the antibodies and the promoter regions indicated. (C) Luciferase assays (mean ± SD) with the indicated reporter plasmids in SK and SK-TRβ cells cotransfected with siControl or siNCoR in the presence of an expression vector for NCoR (CMV-NCoR) or the empty vector (CMV). Luciferase activity (means ± SD) was determined in cells treated with and without T3.