Fig. 4.

(A) Raji-luciferase cells (1 × 10⁶) were injected into the tail vein of NOD/SCID mice (n = 5). Mice were treated with PG545 (20 mg/kg once a week) starting 1 d after cell inoculation, and IVIS imaging was applied to quantify tumor load. Homing of Raji cells to the bone marrow was further confirmed by immunostaining of decalcified backbone sections with anti-human HLA that labels human cells (B, Upper). Slides were similarly stained with anti-CD31 Ab that marks blood vessels (B, Lower). (C and D) NOD/SCID mice (n = 5) were inoculated (i.v.) with Raji-luciferase cells, and mice were treated with mAb 9E8 (600 µg/mouse every other day) or mAb H1023 (500 µg/mouse every other day) as single agents (C) or both together (D), and tumor growth was evaluated by IVIS imaging (Left). (Right) Quantification of luciferase signals is shown graphically.