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G2Cre-driven Wt1 deletion. Effective deletion of Wt1 in the G2-Gata4 lineage is confirmed by the absence of WT1 protein (compare A and C) and by the decrease in the RALDH2 enzyme, a known direct Wt1 target (compare B and D). ENDO, endocardium; EPI, epicardium; V, ventricle. (Scale bars: 50 µm.)
