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. 2016 Jan 4;113(3):E319–E327. doi: 10.1073/pnas.1510518113

Fig. 4.

Fig. 4.

Combination therapy with vaccination induces robust tumor destruction and effector T-cell infiltration into the tumor. (A–H) TUBO mammary carcinoma cells were implanted into the flanks of female BALB/c mice on day 0. Tumor-bearing mice were given control rat IgG on days 10 and 14 (A and E), vaccine [anti–DEC-205/HER2/poly(I:C)] on days 10 and 14 (B and F), combination immunotherapy with aOX40 on days 10 and 14/aCTLA-4 on days 10, 12, and 14 (C and G), or both vaccine and combination immunotherapy (D and H). Tumors were harvested on day 21 and were analyzed by immunohistochemistry. Images depict sections of paraffin-embedded slides stained using H&E (A–D) or anti-CD3 plus DAPI (E–H). (I and J) Tumors were harvested and analyzed by flow cytometry. Graphs depict the mean ± SEM for one of two independent experiments (n = 4 or 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.