Fig. 4.

miR-276 up-regulates brm expression by direct targeting. (A) Luciferase reporter assays in S2 cells cotransfected with miR-276 overexpression vectors and psi-CHECK2 vectors containing wild (WT) or mutant (MT) sequences of target genes (n = 6). (B) Target verification by RIP analysis using the anti-Ago1 antibody in the ovaries of locusts injected with agomir-276 or agomir-ck (n = 4). (C–F) The protein (C and D) and mRNA (E and F) expression changes of brm and lok after miR-276 inhibition in gregarious or overexpression in solitarious ovaries (n = 5). (G and H) The protein (G) and mRNA (H) levels of brm in ovaries and eggs of gregarious (G) and solitarious (S) locusts (n = 5). The data are shown as mean ± SEM, *P < 0.05, **P < 0.01.