Fig. S1.

Sixty percent hatching time analysis. (A) Hatching time for 60% eggs from gregarious locusts (G) was significantly shorter than that from solitarious locusts (S) (n = 40). (B–E) Hatching time for 60% eggs was extended by miR-276 inhibition in the gregarious females (B), shortened by miR-276 overexpression in the solitarious females (C), extended by brm knockdown in the gregarious females (D), and delayed by brm knockdown in the solitarious females pretreated with agomir-276 (E) (n = 25). The data are shown as mean ± SEM, *P < 0.05, **P < 0.01.