Fig. S3.

Interaction between miR-276 and its putative targets. (A) Results of luciferase vectors carrying target site cotransfection with miR-276 overexpression vector compared with miRNA control (miR-ck) overexpression vector in S2 cells (n = 6). The data are shown as mean ± SEM, *P < 0.05, **P < 0.01. (B) Sequence alignment of miR-276 with the predicted target sites in the 3′ UTR of usp, tsr, lok, and syx1a and the coding region of brm. The point mutations (red) in the putative target sites were engineered in the region complementary to the miR-276 seed sequence (purple). The WT sequences of those point mutations are indicated in blue.