Skip to main content
. 2016 Jan 4;113(3):E348–E357. doi: 10.1073/pnas.1523321113

Fig. 5.

Fig. 5.

Δesx-3 and ΔesxH are severely attenuated in aerosol infection of C57BL/6 mice, but Δpe5–ppe4 and ΔmbtB remain virulent. (A and B) Bacterial burdens in lungs (A) and spleens (B) were determined at the indicated time points after aerosol infection with H37Rv, Δesx-3 (mc27788), or Δesx-3::esx-3TB (mc27827). n = 4 or 5 mice per group, except n = 3 for H37Rv-infected spleens at 4 wk. Data points indicate mean ± SEM. Data points that are significantly different (P < 0.05) from H37Rv WT using an unpaired Student’s t test are indicated by an asterisk. (C and D) Following aerosol infection with the indicated strains, bacterial burdens were determined at 24 h and at 3, 8, and 16 wk postinfection in lungs (C) and at 8 and 16 wk postinfection in spleens (D). Strains were H37Rv, ΔmbtB (mc27850), ΔesxH (mc27846), ΔesxH::esxGH (mc27867), Δpe5–ppe4 (mc27848), and Δpe5–ppe4::pe5–ppe4 (mc27868); all strains were cultured in 7H9 medium supplemented with 100 μM hemin before use in aerosol infections. Individual data points are shown; lines indicate mean ± SEM. n = 3 or 4 mice per group. Dotted lines indicate approximate limits of detection.