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. 2016 Jan 5;113(3):E291–E299. doi: 10.1073/pnas.1518634113

Fig. 6.

Fig. 6.

GET of nucleic acids. (A) Schematic of the LK15 proteins engineered to bind to and transduce nucleic acids. (B) Transfection of human mesenchymal stem cells using GET-LK15. Initially we assessed binding capacity of LK15 peptides for plasmid (p)DNA (SIN GFP, to express GFP on transfection), modified synthetic messenger RNA (modRNA) (Miltenyi Biotech; to express GFP on transfection), and small inhibitory (si)RNAs (labeled with FAM fluorophore to detect delivery). After optimizing ratios, we transfected human mesenchymal stem cells with P21-LK15-8R and pDNA (10 µg), modRNA (10 µg), or siRNA (1 µg) and visualized transfection by fluorescence microscopy. (Scale bar, 100 µm.) (C) Quantification of GET-LK15 transfection of human mesenchymal stem cells by flow cytometry (percentage transfection efficiency or relative fluorescence for siRNA) compared with LIPO2000 as a commercial standard. Error bars indicate SD.