Table 1.

RNA binding and ATPase activity of WT RIG-I and Helicase-RD

	WT RIG-I		Helicase-RD
RNA ligand	Kd,app, nM	katpase, s−1	Kd,app, nM	katpase, s−1
5′OH HP RNA	38.5 ± 4	49 ± 1	0.4 ± 0.3	63 ± 1
5′ppp HP RNA	1.8 ± 0.9	33 ± 0.9	0.75 ± 0.3	48 ± 1
Cap-0 HP RNA	1.7 ± 0.5	25 ± 0.4	0.08 ± 0.04	36 ± 0.4
5′ppp ssRNA	N.B.	N.B.	35 ± 5.5	0.9 ± 0.03
Cap-0 ssRNA	N.B.	N.B.	14 ± 4.4	0.3 ± 0.03

N.B., no binding detected.