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. 2015 Dec 31;113(3):644–649. doi: 10.1073/pnas.1517112113

Fig. 1.

Fig. 1.

FAM129B promotes EGF-induced H-Ras activation. (A) U251, LN229, and PC14 cells with or without stable expression of the indicated FAM129B shRNA or a control shRNA were treated with EGF (100 ng/mL) for the indicated time intervals. H-Ras activity was determined by using GST-Raf-RBD to pulldown GTP–H-Ras from cell lysates. Immunoblotting analyses were performed with the indicated antibodies. (B) U251 and LN229 cells with or without stable expression of FAM129B shRNA or a control shRNA were treated with EGF (100 ng/mL) for the indicated time intervals. Immunoblotting analyses were performed with the indicated antibodies. (C) The activities of H-Ras and K-Ras were determined in U87/EGFRvIII cells with or without expression of FAM129B shRNA or a control shRNA. Immunoblotting analyses were performed with the indicated antibodies.