Skip to main content
. 2015 Dec 31;113(3):644–649. doi: 10.1073/pnas.1517112113

Fig. S3.

Fig. S3.

FAM129B Y593 phosphorylation reduces binding of p120-RasGAP to H-Ras. (A) U251 cells depleted or not depleted of FAM129B were treated with EGF (100 ng/mL) for 15 min. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. (B) U251 cells were treated with EGF (100 ng/mL) for 15 min. SOS was immunoprecipitated and its activity was measured. Data represent the mean ± SD of three independent experiments. (C) U87/EGFRvIII cells depleted or not depleted of FAM129B and with reconstituted expression of WT rFAM129B or rFAM129B Y593F mutant were analyzed by immunoprecipitation and immunoblotting analyses with the indicated antibodies. (D) Purified His-p120-RasGAP and GDP-bound GST–H-Ras in the presence or absence of tetrafluoroaluminate (AlF4−; 10 mM NaF and 30 μM AlCl3) were incubated with or without GST–EGFR-phosphorylated or nonphosphorylated S-FAM129B. A His-pulldown assay was performed.