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. 2015 Dec 30;113(3):E396–E405. doi: 10.1073/pnas.1512779113

Fig. S4.

Fig. S4.

Interaction of PYR1 and CAR1 generates punctate/globular structures in plasma membrane. Confocal images of transiently transformed tobacco epidermal cells coexpressing CAR1-YFPN/YFPC-PYR1 interacting proteins and a key cellular component for membrane trafficking. Asterisks indicate the presence of CAR1-PYR1 in membrane complexes decorated by At1g20110. To investigate the interaction of CAR1 and PYR1, we used the pSPYNE-35S and pYFPC43 vectors, respectively. At1g20110 was recombined by LR reaction into pH7WGR2 vector to coexpress RFP-At1g20110 together with CAR1-YFPN/YFPC-PYR1 using Agrobacterium-mediated transfection of tobacco leaves.