FIGURE 3.

(A) Schematic representation of the specialized transducing phage. A replicating shuttle phasmid phAE2067 containing mabA, inhA carrying the S94A mutation, a hyg resistance cassette, and hemZ was used to transduce M. tuberculosis (M. tb). The two possible sites of recombination are marked 1 and 2. (B) The recombination can occur either before the point mutation (crossover type 1), resulting in an INH-resistant and ETH-resistant recombinant carrying the S94A mutation, or after the point mutation (crossover type 2; the strain contains a wild-type inhAgene). (C) Individual M. tuberculosis H37Rv inhA(S94A) transductants (n= 150) were screened by picking and patching onto plates containing either hygromycin (50 μg/ml) or INH (0.2 μg/ml). Reprinted with permission.doi:10.1128/microbiolspec.MGM2-0037-2013.f3