Table 1.

Schedule of stressor used in the 35-day CUS procedure

Day	Stressor
1	Restraint stress (4 hours)
2	Shaking (1 hour)
3	Tail pinch (10 minutes)
4	Swim stress (12°C, 10 minutes)
5	Constant light (24 hours)
6	Cold swim (18°C, 10 minutes)
7	Wet bedding (24 hours)
8	Water deprivation (12 hours)
9	Social isolation (24 hours)
10	Swim stress (12°C, 10 minutes)
11	Tail pinch (10 minutes)
12	Water deprivation (12 hours)
13	Shaking (1 hour)
14	Hot water stress at 42°C (5 minutes)
15	Restraint stress (4 hours)
16	Shaking (1 hour)
17	Constant light (24 hours)
18	Social isolation (24 hours)
19	Swim stress at 12°C (10 minutes)
20	Water deprivation (12 hours)
21	Wet bedding (24 hours)
22	Tail pinch (10 minutes)
23	Hot water stress at 42°C (5 minutes)
24	Restraint stress (4 hours)
25	Water deprivation (12 hours)
26	Constant light (24 hours)
27	Swim stress (12°C, 10 minutes)
28	Open field test (5 minutes)
29	Wet bedding (24 hours)
30	Hot water stress at 42°C (5 minutes)
31	Restraint stress (4 hours)
32	Tail pinch (10 minutes)
33	Water deprivation (12 hours)
34	Constant light (24 hours)
35	Shaking (1 hour)

Abbreviation: CUS, chronic unpredictable stress.