Figure 5. Effect of CAA on growth and viability of wild-type, mABH2^−/− and ANPG^−/− MEF cells.

Wild-type cells, mABH2^−/− and ANPG^−/− cells were used at passages 16 to 34. Cells were seeded in 96-well plates with approximately 3000 cells/well. After 22 hours the cells were treated with 0-20 μM CAA for 2 hours, washed twice with PBS, before fresh media was added. Cell survival was measured after 48 h. Results are expressed as cell numbers in CAA-treated cultures compared to untreated controls. Cell numbers were measured using the MTT assay (Roche). Untreated wild-type, mABH2^−/− (A) and ANPG^−/− (B) cell lines had a density of about 60% after 48 hours. Values presented are average of five to ten independent experiments, and the standard deviations are always less than 10%.