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. 2016 Jan 25;11(1):e0147886. doi: 10.1371/journal.pone.0147886

Fig 4. Effect of TSA on the activities of wild type and Sp(-40/-31) mutated ek1 minimal promoters.

Fig 4

A. Promoter activity of pGL4.10-ek1(-69/+1) reporter construct in HCT116 cells treated with the indicated concentrations of TSA for 24 hours (*p < 0.001; **p < 0.01; ***p < 0.05 vs. DMSO control; #p < 0.05, significant within TSA treatment group). B. Promoter activity of pGL4.10-ek1(-69/+1) reporter construct in HCT116 cells treated with 1 μM of TSA for the indicated time points (*p < 0.05 vs. DMSO control). C. Activities of wild type ek1 minimal promoter and Sp(-40/-31)-mutated ek1 minimal promoter after treatment with 1 μM of TSA for 24 hours. Each bar represents the mean ± SEM of triplicate samples from three independent experiments. D. Effect of TSA on ek1 gene expression in HCT116 cells. HCT116 cells were treated with 1 μM of TSA for 24 hours (**p < 0.01 vs. DMSO control). Each bar represents the mean ± SEM of triplicate samples from three independent experiments.