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. 2016 Jan 13;6:19229. doi: 10.1038/srep19229

Figure 1. FAS rs2234767 A and rs1800682 G alleles affect coupled SP1 and STAT1 recruitment to chromatin.

Figure 1

(A) Chromatin immunoprecipitation (ChIP) of the FAS promoter with three different genotypes (rs2234767 GG/rs1800682 AA, rs2234767 GA/rs1800682 AG and rs2234767 AA/rs1800682 GG) using antibody for SP1 and STAT1(single pool generated from triplicate biological samples/manipulation; triplicate measurements/pool; mean ± SE). (B) Sequential ChIP of the FAS promoter containing SP1 motif immunoprecipitated first using antibody for SP1 followed by antibody for STAT1 (upper) or first using antibody for STAT1 followed by antibody for SP1 (lower). (C) Sequential ChIP of the FAS promoter containing STAT1 motif immunoprecipitated first using antibody for SP1 followed by antibody for STAT1 (upper) or first using antibody for STAT1 followed by antibody for SP1 (lower). For all combined genotypes in the figure, left genotype arises from the rs2234767 and right from the rs1800682 polymorphism.