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. 2016 Jan 25;11(1):e0147192. doi: 10.1371/journal.pone.0147192

Fig 1. Overexpression of M. bovis BCG lipoproteins increases HIV susceptibility of M. smegmatis-infected human CD4+ T cells.

Fig 1

A. Levels of expression of M. bovis BCG lipoprotein genes in various M. smegmatis knock-in strains. Cycle threshold values were first normalized to the housekeeping gene, sigA, and expressed as fold increase relative to wild-type M. bovis BCG. Each cycle difference was assumed to represent a 2-fold difference in gene expression. The data are representative of three independent experiments. B. The percentage of X4-tropic, GFP-expressing pseudovirus-infected CD4+ cells without any stimulation (Unstim) or after infection with M. bovis BCG Copenhagen (BCG), M. smegmatis MC2155 containing empty vector (M. smeg), or M. smegmatis strains overexpressing BCG lipoproteins PhoS1, LprQ, LprD, LprI, LprH, LprF, LppX, LprP, or MPT83. C. The TLR2 agonist zymosan (Zym), TLR2 and TLR4 antagonist OxPAPC, or the TLR4-specific antagonist CLI-095 was added to PBMC prior to HIV infection. Results represent combined flow cytometric analysis of CD4+ cells after infection with an X4-tropic GFP+ pseudovirus, from three independent experiments. * p< 0.05; ** p< 0.005.