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. 2016 Jan 25;11(1):e0147192. doi: 10.1371/journal.pone.0147192

Table 1. List of gene-specific primer pairs used to generate mycobacterial recombinant strains.

Gene Fwd Primer Rv Primer
phoS1 gatacgtcgccggactgt gtcaacgaggctagctggaa
lppX gtgagcgcaaatgaatgatg aattttcagatgccgtcagg
mpt83 actccgaatcactggtgtcc gtgatctatcggcacgaagc
lprH ctcgttgctggctcgatac ccgtgggacacatttgagta
lprD cacctggggtattaggcaag ggtgcgaatcctctcgac
lprF atggacggggctggattc ggttcgcgcctgttatcc
lprI accagtctgagccctgtgg gcacgcgctaaagatattgc
lprQ tgcatgttgtagctgcgttt ccttgaccagatcgtcatcc
lprP acccgaactatcacccatga gatcggatggccttttgtt

All primers are designed to function in the Gateway system (start with GGGG-AttB1-MCS-gene specific primer).