Table 1. List of gene-specific primer pairs used to generate mycobacterial recombinant strains.
Gene | Fwd Primer | Rv Primer |
---|---|---|
phoS1 | gatacgtcgccggactgt | gtcaacgaggctagctggaa |
lppX | gtgagcgcaaatgaatgatg | aattttcagatgccgtcagg |
mpt83 | actccgaatcactggtgtcc | gtgatctatcggcacgaagc |
lprH | ctcgttgctggctcgatac | ccgtgggacacatttgagta |
lprD | cacctggggtattaggcaag | ggtgcgaatcctctcgac |
lprF | atggacggggctggattc | ggttcgcgcctgttatcc |
lprI | accagtctgagccctgtgg | gcacgcgctaaagatattgc |
lprQ | tgcatgttgtagctgcgttt | ccttgaccagatcgtcatcc |
lprP | acccgaactatcacccatga | gatcggatggccttttgtt |
All primers are designed to function in the Gateway system (start with GGGG-AttB1-MCS-gene specific primer).