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. 2016 Jan 25;11(1):e0147618. doi: 10.1371/journal.pone.0147618

Fig 3. Effects of Lysine or Leucine deficient media on the MRFs genes expression in gilthead sea bream cultured muscle cells.

Fig 3

Quantitative relative expression of (A) Pax7, (B) Myf5, (C) Myogenin, (D) MRF4, (E) MyoD1 and (F) MyoD2 normalized to the geometric mean of EF1α and RPS18 in myocytes at days 2, 4 or 8 after incubation with a growth medium control or deficient in Lys or Leu. Data are shown as means ± SEM (n = 3–4). Asterisks indicate significant differences compared to the control at each time (p<0.05). Different letters indicate differences for each group throughout the culture (p<0.05).