Table 1. Primers used in the qPCR analyses.
| Gene | Primer sequences (5’-3’) | Ta (°C) | Accession No. |
|---|---|---|---|
| EF1a | F:CTTCAACGCTCAGGTCATCAT R:GCACAGCGAAACGACCAAGGGGA | 60 | AF184170 |
| RPL27 | F:AAGAGGAACACAACTCACTGCCCCAC R:GCTTGCCTTTGCCCAGAACTTTGTAG | 68 | AY188520 |
| RPS18 | F:GGGTGTTGGCAGACGTTAC R:CTTCTGCCTGTTGAGGAACCA | 60 | AM490061.1 |
| IGF-I | F:ACAGAATGTAGGGACGGAGCGAATGGAC R:TTCGGACCATTGTTAGCCTCCTCTCTG | 60 | AY996779 EF688015 EF688016 |
| IGF-II | F:TGGGATCGTAGAGGAGTGTTGT R:CTGTAGAGAGGTGGCCGACA | 60 | AY996778 |
| IGF-IRa | F:AGCATCAAAGACGAACTGG R:CTCCTCGCTGTAGAAGAAGC | 55 | KT156846 |
| IGF-IRb | F:GCTAATGCGAATGTGTTGG R:CGTCCTTTATGCTGCTGATG | 55 | KT156847 |
| IGFBP-4 | F:TCCACAAACCAGAGAAGCAA R:GGGTATGGGGATTGTGAAGA | 60 | F5T95CD02JMZ9K |
| IGFBP-5 | F:TTTCTCTCTCGGTGTGC R:TCAAGTATCGGCTCCAG | 60 | AM963285 |
| Pax7 | F:ATGAACACTGTCGGCAACG R:AGGCTGTCCACACTCTTGATG | 64 | JN034418 |
| Myf5 | F:CTACGAGAGCAGGTGGAGAACT R:TGTCTTATCGCCCAAAGTGTC | 64 | JN034420 |
| Myogenin | F:CAGAGGCTGCCCAAGGTCGAG R:CAGGTGCTGCCCGAACTGGGCTCG | 68 | EF462191 |
| MRF4 | F:CATCCCACAGCTTTAAAGGCA R:GAGGACGCCGAAGATTCACT | 60 | JN034421 |
| MyoD1 | F:TTTGAGGACCTGGACCC R:CTTCTGCGTGGTGATGGA | 60 | AF478568.1 |
| MyoD2 | F:CACTACAGCGGGGATTCAGAC R:CGTTTGCTTCTCCTGGACTC | 60 | AF478569 |
| PCNA | F:TGTTTGAGGCACGTCTGGTT R:TGGCTAGGTTTCTGTCGC | 58 | NM_131404.2 |
| MHC | F:AGCAGATCAAGAGGAACAGCC R:GACTCAGAAGCCTGGCGATT | 58 | AY550963.1 |
| AKT2 | F:GCTCACCCCACTCTTCAGAC R:AAATTGGGAAATGTGCTTGC | 60 | ERA047531 |
| ERK2 | F:AAAGCTCTGGACCTGTTGGA R:TCATCCAGCTCCATGTCAAA | 60 | ERA047531 |
| TOR | F:CAGACTGACGAGGATGCTGA R:AGTTGAGCAGCGGGTCATAG | 60 | --- |
| FOXO3 | F:CAGCAGCCTGGAGTGTGATA R:CCAGCTCTGAGAGGTCTGCT | 60 | --- |
| 4EBP1 | F:CCAACCTGCGACTCATCTCT R:GTTCCTCTCATCCTCCCACA | 60 | --- |
| 70S6K | F:GCACCAGAAAGGCATCATCT R:AAGGTGTGGGTCACTGTTCC | 60 | --- |
| ATF4 | F:TCGCTCGATTTGCCGAAATG R:TGGCTGGATGCACTGTTTTG | 60 | JQ308824.1 |
| AS | F:ACTGCTGTTTTGGCTTCCAC R:ACTTCTTGATGCGCAAAGGC | 58 | --- |
| CHOP | F:AAGAAGTCGGTGGACAGGTTC R:AGTTGCGCATCTTGGCTTTG | 58 | --- |
F: forward; R: reverse; Ta: annealing temperature.