Table 1. Determination of diffusion state of GFP-TDP35 as a mimic of TDP43 CTF by caspase 3 using FCS measurements.

	CPM (kHz)	DFast (μm2/s)	Fast component (%)	DSlow (μm2/s)	Slow component (%)	Mw from DFast (kDa)	Cell number
GFP-TDP35	3.02 ± 0.396n.s.	12.5 ± 2.30***	59.7 ± 3.50***	0.324 ± 0.756***	40.3 ± 3.50***	1122	13
GFP	3.28 ± 0.412	43.3 ± 6.74	99.1 ± 0.315	0.0486 ± 0.0545	0.896 ± 0.315	27†	8

Each values indicates mean ± S.D. Measured cell number was indicated (Cell number). ^†Denotes as a basis for calculation of molecular weight from diffusion coefficient. Significance between GFP-TDP35 and GFP was tested by Student’s t test: ***p < 0.001 and n.s.: not significant.