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. 2016 Jan 13;6:19409. doi: 10.1038/srep19409

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Aqueous films were measured on the surface of ‘rough’ (a) and ‘smooth’ (b) PSM, using 3D scanning laser microscopy, with a detection limit of ≈2 μm. Three random PSM areas of 200 μm x 289 μm were analyzed. For each area, aqueous film data from ten equidistant x-axis transects were pooled, and the probability function was estimated using kernel density estimation. The measurements at different matric potential values were done sequentially.