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. 2016 Jan 14;6:19296. doi: 10.1038/srep19296

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(A) The overexpression of Cdo1in mBMSCs was confirmed by RT-PCR. (B) The overexpression of Cdo1in mBMSCs was confirmed by Western blot. (C) Overexpression of Cdo1 inhibited mRNA expression levels of Col1a1 and Ibsp stimulated by osteogenic induction. (D) Overexpression of Cdo1impaired the ALP staining after 7 days of osteogenic induction. (E) Overexpression of Cdo1inhibited the ALP activity after 7 days of osteogenic induction as determined by quantitative ALP activity assay. (F) Overexpression of Cdo1 reduced mineralization post-14 days of osteogenic induction. (G) Quantification of ARS staining in (F). *p < 0.05.

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